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plasmids pgc-fu-3flag-cbh-gcgfp-ires-puromycin  (Genechem)

 
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    Structured Review

    Genechem plasmids pgc-fu-3flag-cbh-gcgfp-ires-puromycin
    Plasmids Pgc Fu 3flag Cbh Gcgfp Ires Puromycin, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pgc-fu/gv314+cmv+3flag+gfp/pmc11970384-161-0-3
    Average 90 stars, based on 1 article reviews
    plasmids pgc-fu-3flag-cbh-gcgfp-ires-puromycin - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells
    Article Snippet: Lentivirus packaging system, including plasmid pGC-FU, pHelper 1.0, pHelper 2.0 and plasmid Foxc2, was purchased from Shanghai GeneChem Co., China.

    Article Title: Lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase driven by the hTERT promoter combined with gemcitabine: a potential strategy for cancer therapy.
    Article Snippet: Dm-dNK-3Flag was amplified by PCR and ligated into plasmid PGC-FU (GeneChem, Shanghai, China) consisting of a 5'-long terminal repeat (LTR), cytomegalovirus (CMV) promoter, multiple clone site, a green fluorescent protein (GFP) sequence and a 3'-LTR.

    Article Title: Cytotoxic effects of adenovirus- and lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase on Bcap37 breast cancer cells.
    Article Snippet: The coding sequence of Dm-dNK was released from plasmid PGEM-T-dNK with endonucleases EcoRI and BamHI (New England Biolabs, Beverley, MA) and cloned in-frame into plasmid Pentr13 [containing the sequence of a mouse CMV promoter, His-tag(N) and multiple cloning sites] and plasmid PGC-FU [Genechem, Shanghai, China; containing the sequence of a mouse CMV promoter, multiple cloning sites and a green fluorescent protein (GFP) sequence] to generate PENTR13-dNK and PGC-FU-dNK, respectively.

    Plasmid Preparation:

    Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells
    Article Snippet: Lentivirus packaging system, including plasmid pGC-FU, pHelper 1.0, pHelper 2.0 and plasmid Foxc2, was purchased from Shanghai GeneChem Co., China.

    Article Title: Lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase driven by the hTERT promoter combined with gemcitabine: a potential strategy for cancer therapy.
    Article Snippet: Dm-dNK-3Flag was amplified by PCR and ligated into plasmid PGC-FU (GeneChem, Shanghai, China) consisting of a 5'-long terminal repeat (LTR), cytomegalovirus (CMV) promoter, multiple clone site, a green fluorescent protein (GFP) sequence and a 3'-LTR.

    Article Title: Cytotoxic effects of adenovirus- and lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase on Bcap37 breast cancer cells.
    Article Snippet: The coding sequence of Dm-dNK was released from plasmid PGEM-T-dNK with endonucleases EcoRI and BamHI (New England Biolabs, Beverley, MA) and cloned in-frame into plasmid Pentr13 [containing the sequence of a mouse CMV promoter, His-tag(N) and multiple cloning sites] and plasmid PGC-FU [Genechem, Shanghai, China; containing the sequence of a mouse CMV promoter, multiple cloning sites and a green fluorescent protein (GFP) sequence] to generate PENTR13-dNK and PGC-FU-dNK, respectively.

    Clone Assay:

    Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells
    Article Snippet: Lentivirus packaging system, including plasmid pGC-FU, pHelper 1.0, pHelper 2.0 and plasmid Foxc2, was purchased from Shanghai GeneChem Co., China.

    Article Title: Lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase driven by the hTERT promoter combined with gemcitabine: a potential strategy for cancer therapy.
    Article Snippet: Dm-dNK-3Flag was amplified by PCR and ligated into plasmid PGC-FU (GeneChem, Shanghai, China) consisting of a 5'-long terminal repeat (LTR), cytomegalovirus (CMV) promoter, multiple clone site, a green fluorescent protein (GFP) sequence and a 3'-LTR.

    Article Title: Cytotoxic effects of adenovirus- and lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase on Bcap37 breast cancer cells.
    Article Snippet: The coding sequence of Dm-dNK was released from plasmid PGEM-T-dNK with endonucleases EcoRI and BamHI (New England Biolabs, Beverley, MA) and cloned in-frame into plasmid Pentr13 [containing the sequence of a mouse CMV promoter, His-tag(N) and multiple cloning sites] and plasmid PGC-FU [Genechem, Shanghai, China; containing the sequence of a mouse CMV promoter, multiple cloning sites and a green fluorescent protein (GFP) sequence] to generate PENTR13-dNK and PGC-FU-dNK, respectively.

    Cloning:

    Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells
    Article Snippet: Lentivirus packaging system, including plasmid pGC-FU, pHelper 1.0, pHelper 2.0 and plasmid Foxc2, was purchased from Shanghai GeneChem Co., China.

    Article Title: Lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase driven by the hTERT promoter combined with gemcitabine: a potential strategy for cancer therapy.
    Article Snippet: Dm-dNK-3Flag was amplified by PCR and ligated into plasmid PGC-FU (GeneChem, Shanghai, China) consisting of a 5'-long terminal repeat (LTR), cytomegalovirus (CMV) promoter, multiple clone site, a green fluorescent protein (GFP) sequence and a 3'-LTR.

    Article Title: Cytotoxic effects of adenovirus- and lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase on Bcap37 breast cancer cells.
    Article Snippet: The coding sequence of Dm-dNK was released from plasmid PGEM-T-dNK with endonucleases EcoRI and BamHI (New England Biolabs, Beverley, MA) and cloned in-frame into plasmid Pentr13 [containing the sequence of a mouse CMV promoter, His-tag(N) and multiple cloning sites] and plasmid PGC-FU [Genechem, Shanghai, China; containing the sequence of a mouse CMV promoter, multiple cloning sites and a green fluorescent protein (GFP) sequence] to generate PENTR13-dNK and PGC-FU-dNK, respectively.

    Pyrolysis Gas Chromatography:

    Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells
    Article Snippet: Lentivirus packaging system, including plasmid pGC-FU, pHelper 1.0, pHelper 2.0 and plasmid Foxc2, was purchased from Shanghai GeneChem Co., China.

    Article Title: Lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase driven by the hTERT promoter combined with gemcitabine: a potential strategy for cancer therapy.
    Article Snippet: Dm-dNK-3Flag was amplified by PCR and ligated into plasmid PGC-FU (GeneChem, Shanghai, China) consisting of a 5'-long terminal repeat (LTR), cytomegalovirus (CMV) promoter, multiple clone site, a green fluorescent protein (GFP) sequence and a 3'-LTR.

    Article Title: Cytotoxic effects of adenovirus- and lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase on Bcap37 breast cancer cells.
    Article Snippet: The coding sequence of Dm-dNK was released from plasmid PGEM-T-dNK with endonucleases EcoRI and BamHI (New England Biolabs, Beverley, MA) and cloned in-frame into plasmid Pentr13 [containing the sequence of a mouse CMV promoter, His-tag(N) and multiple cloning sites] and plasmid PGC-FU [Genechem, Shanghai, China; containing the sequence of a mouse CMV promoter, multiple cloning sites and a green fluorescent protein (GFP) sequence] to generate PENTR13-dNK and PGC-FU-dNK, respectively.

    Amplification:

    Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells
    Article Snippet: Lentivirus packaging system, including plasmid pGC-FU, pHelper 1.0, pHelper 2.0 and plasmid Foxc2, was purchased from Shanghai GeneChem Co., China.

    Article Title: Lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase driven by the hTERT promoter combined with gemcitabine: a potential strategy for cancer therapy.
    Article Snippet: Dm-dNK-3Flag was amplified by PCR and ligated into plasmid PGC-FU (GeneChem, Shanghai, China) consisting of a 5'-long terminal repeat (LTR), cytomegalovirus (CMV) promoter, multiple clone site, a green fluorescent protein (GFP) sequence and a 3'-LTR.

    Article Title: Cytotoxic effects of adenovirus- and lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase on Bcap37 breast cancer cells.
    Article Snippet: The coding sequence of Dm-dNK was released from plasmid PGEM-T-dNK with endonucleases EcoRI and BamHI (New England Biolabs, Beverley, MA) and cloned in-frame into plasmid Pentr13 [containing the sequence of a mouse CMV promoter, His-tag(N) and multiple cloning sites] and plasmid PGC-FU [Genechem, Shanghai, China; containing the sequence of a mouse CMV promoter, multiple cloning sites and a green fluorescent protein (GFP) sequence] to generate PENTR13-dNK and PGC-FU-dNK, respectively.

    Polymerase Chain Reaction:

    Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells
    Article Snippet: Lentivirus packaging system, including plasmid pGC-FU, pHelper 1.0, pHelper 2.0 and plasmid Foxc2, was purchased from Shanghai GeneChem Co., China.

    Article Title: Lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase driven by the hTERT promoter combined with gemcitabine: a potential strategy for cancer therapy.
    Article Snippet: Dm-dNK-3Flag was amplified by PCR and ligated into plasmid PGC-FU (GeneChem, Shanghai, China) consisting of a 5'-long terminal repeat (LTR), cytomegalovirus (CMV) promoter, multiple clone site, a green fluorescent protein (GFP) sequence and a 3'-LTR.

    Article Title: Cytotoxic effects of adenovirus- and lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase on Bcap37 breast cancer cells.
    Article Snippet: The coding sequence of Dm-dNK was released from plasmid PGEM-T-dNK with endonucleases EcoRI and BamHI (New England Biolabs, Beverley, MA) and cloned in-frame into plasmid Pentr13 [containing the sequence of a mouse CMV promoter, His-tag(N) and multiple cloning sites] and plasmid PGC-FU [Genechem, Shanghai, China; containing the sequence of a mouse CMV promoter, multiple cloning sites and a green fluorescent protein (GFP) sequence] to generate PENTR13-dNK and PGC-FU-dNK, respectively.



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